View source for Journal:Fast detection of 10 cannabinoids by RP-HPLC-UV method in Cannabis sativa L.

{{DISPLAYTITLE:Journal:Fast detection of 10 cannabinoids by RP-HPLC-UV method in ''Cannabis sativa'' L.}}
{{Infobox journal article
|name         = 
|image        = 
|alt          = <!-- Alternative text for images -->
|caption      = 
|title_full   = Fast detection of 10 cannabinoids by RP-HPLC-UV method in ''Cannabis sativa'' L.
|journal      = ''Molecules''
|authors      = Mandrioli, Mara; Tura, Matilde; Scotti, Stefano; Toschi, Tullia Gallina
|affiliations = University of Bologna, Shimadzu Italia
|contact      = Email: tullia dot gallinatoschi at unibo dot it
|editors      = Locatelli, Marcello
|pub_year     = 2019
|vol_iss      = '''24'''(11)
|pages        = 2113
|doi          = [http://doi.org/10.3390/molecules24112113 10.3390/molecules24112113]
|issn         = 1420-3049
|license      = [http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International]
|website      = [https://www.mdpi.com/1420-3049/24/11/2113/htm https://www.mdpi.com/1420-3049/24/11/2113/htm]
|download     = [https://www.mdpi.com/1420-3049/24/11/2113/pdf https://www.mdpi.com/1420-3049/24/11/2113/pdf] (PDF)
}}

==Abstract==
[[wikipedia:Cannabis sativa|Cannabis]] has regained much attention as a result of updated legislation authorizing many different uses, and it can be classified on the basis of the content of [[wikipedia:Tetrahydrocannabinol|Δ9-tetrahydrocannabinol]] (Δ9-THC), a psychotropic substance for which there are legal limitations in many countries. For this purpose, accurate qualitative and quantitative determination is essential. The relationship between THC and [[wikipedia:Cannabidiol|cannabidiol]] (CBD) is also significant, as the latter substance is endowed with many specific and non-psychoactive proprieties. For these reasons, it becomes increasingly important and urgent to utilize fast, easy, validated, and harmonized procedures for determination of [[wikipedia:Cannabinoid|cannabinoids]]. The procedure described herein allows rapid determination of 10 cannabinoids from the [[wikipedia:Inflorescence|inflorescences]] of ''Cannabis sativa'' L. by extraction with organic solvents. Separation and subsequent detection are by [[wikipedia:Reversed-phase chromatography|reversed-phase]] [[high-performance liquid chromatography]] with ultraviolet detector (RP-HPLC-UV). Quantification is performed by an external standard method through the construction of calibration curves using pure standard [[Chromatography|chromatographic]] reference compounds. The main cannabinoids dosed (g/100 g) in actual samples were cannabidiolic acid (CBDA), CBD, and Δ9-THC (Sample L11 CBDA 0.88 ± 0.04, CBD 0.48 ± 0.02, Δ9-THC 0.06 ± 0.00; Sample L5 CBDA 0.93 ± 0.06, CBD 0.45 ± 0.03, Δ9-THC 0.06 ± 0.00). The present validated RP-HPLC-UV method allows determination of the main cannabinoids in ''Cannabis sativa'' L. inflorescences and appropriate legal classification of it as either [[wikipedia:Hemp|hemp]] or a [[wikipedia:Cannabis (drug)|drug-type]].

'''Keywords''': cannabinoids, ''Cannabis sativa'' L., HPLC, validation




==Supplementary material==
The following are available [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600594/bin/molecules-24-02113-s001.zip online here] (.zip):

File S1: Standard operating procedure (SOP) of the method presented in this article, Table S1: Calibration curves relating to the standard solution of 10 cannabinoids determined by RP-HPLC-UV method, Figure S1: Calibration curves relating to the standard solution of 10 cannabinoids determined by RP-HPLC-UV method, File S2: Preliminary tests carried out for development of the analytical procedure by RP-HPLC-UV

==Acknowledgements==
The authors gratefully acknowledge Enecta Srl for providing samples. The experimentation was conducted in the context of a PhD project entitled ''Harmonized procedures of analysis of medical, herbal, food and industrial cannabis: Development and validation of cannabinoids’ quality control methods, of extraction and preparation of derivatives from the plant raw material, according to the product destination'' and funded by Enecta Srl.

===Author contributions===
Conceptualization, T.G.T., M.M. and M.T.; Methodology, M.M.; Software, M.M. and S.S.; Validation, M.M.; Formal analysis, M.M.; Investigation, T.G.T. and M.M.; Resources, T.G.T. and M.M.; Data curation, M.M., T.G.T. and M.T.; Writing—original draft preparation, M.M., M.T. and T.G.T.; writing—review and editing, T.G.T. and S.S.; Visualization, T.G.T.; Supervision, T.G.T.; project administration, T.G.T.; funding acquisition, T.G.T.

===Funding===
This research received no external funding; this trial received financial support from Enecta Srl.

===Conflicts of interest===
The authors declare no conflict of interest.

==References==
{{Reflist|colwidth=30em}}

==Notes==
This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added. 

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