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<div style="float: left; margin: 0.5em 0.9em 0.4em 0em;">[[File:Fig2 Trellet JOfIntegBioinfo2018 15-2.jpg|240px]]</div>
<div style="float: left; margin: 0.5em 0.9em 0.4em 0em;">[[File:Fig0 Cardenia JofFoodDrugAnal2018 26-4.jpg|240px]]</div>
'''"[[Journal:Semantics for an integrative and immersive pipeline combining visualization and analysis of molecular data|Semantics for an integrative and immersive pipeline combining visualization and analysis of molecular data]]"'''
'''"[[Journal:Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L|Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L]]"'''


The advances made in recent years in the field of structural biology significantly increased the throughput and complexity of data that scientists have to deal with. Combining and [[Data analysis|analyzing]] such heterogeneous amounts of data became a crucial time consumer in the daily tasks of scientists. However, only few efforts have been made to offer scientists an alternative to the standard compartmentalized tools they use to explore their data and that involve a regular back and forth between them. We propose here an integrated pipeline especially designed for immersive environments, promoting direct interactions on semantically linked 2D and 3D heterogeneous data, displayed in a common working space. The creation of a semantic definition describing the content and the context of a molecular scene leads to the creation of an intelligent system where data are (1) combined through pre-existing or inferred links present in our hierarchical definition of the concepts, (2) enriched with suitable and adaptive analyses proposed to the user with respect to the current task and (3) interactively presented in a unique working environment to be explored. ('''[[Journal:Semantics for an integrative and immersive pipeline combining visualization and analysis of molecular data|Full article...]]''')<br />
A routine method for determining [[wikipedia:Cannabinoid|cannabinoids]] in ''Cannabis sativa'' L. [[wikipedia:Inflorescence|inflorescence]], based on fast [[gas chromatography]] coupled to [[mass spectrometry]] (fast GC-MS), was developed and validated. To avoid the [[wikipedia:Decarboxylation|decarboxylation]] of the carboxyl group of cannabinoids, different derivatization approaches—i.e., silylation and esterification (diazomethane-mediated) reagents and solvents (pyridine or ethyl acetate)—were tested. The methylation significantly increased the signal-to-noise ratio of all carboxylic cannabinoids, except for cannabigerolic acid (CBGA). Since [[wikipedia:Diazomethane|diazomethane]] is not commercially available, is considered a hazardous reactive, and requires one-day synthesis by specialized chemical staff, the process of silylation was used along the entire validation of a routine method. The method gave a fast (total analysis time < 7.0 min) and satisfactory resolution (R > 1.1), with a good repeatability (intraday < 8.38%; interday < 11.10%) and sensitivity (LOD < 11.20 ng/mL). ('''[[Journal:Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L|Full article...]]''')<br />
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Revision as of 17:13, 9 December 2019

Fig0 Cardenia JofFoodDrugAnal2018 26-4.jpg

"Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L"

A routine method for determining cannabinoids in Cannabis sativa L. inflorescence, based on fast gas chromatography coupled to mass spectrometry (fast GC-MS), was developed and validated. To avoid the decarboxylation of the carboxyl group of cannabinoids, different derivatization approaches—i.e., silylation and esterification (diazomethane-mediated) reagents and solvents (pyridine or ethyl acetate)—were tested. The methylation significantly increased the signal-to-noise ratio of all carboxylic cannabinoids, except for cannabigerolic acid (CBGA). Since diazomethane is not commercially available, is considered a hazardous reactive, and requires one-day synthesis by specialized chemical staff, the process of silylation was used along the entire validation of a routine method. The method gave a fast (total analysis time < 7.0 min) and satisfactory resolution (R > 1.1), with a good repeatability (intraday < 8.38%; interday < 11.10%) and sensitivity (LOD < 11.20 ng/mL). (Full article...)

Recently featured:

▪ Design and refinement of a data quality assessment workflow for a large pediatric research network
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