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<div style="float: left; margin: 0.5em 0.9em 0.4em 0em;">[[File:Fig2 Palmieri Molecules2019 24-19.png|240px]]</div>
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<div style="float: left; margin: 0.5em 0.9em 0.4em 0em;">[[File:Fig1 Mandrioli Molecules2019 24-11.png|240px]]</div>
'''"[[Journal:Identification of Cannabis sativa L. (hemp) retailers by means of multivariate analysis of cannabinoids|Identification of Cannabis sativa L. (hemp) retailers by means of multivariate analysis of cannabinoids]]"'''
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'''"[[Journal:Fast detection of 10 cannabinoids by RP-HPLC-UV method in Cannabis sativa L.|Fast detection of 10 cannabinoids by RP-HPLC-UV method in Cannabis sativa L.]]"'''
  
In this work, the concentration of nine [[wikipedia:Cannabinoid|cannabinoid]]s—six neutral cannabinoids (THC, CBD, CBC, CBG, CBN, and CBDV) and three acidic cannabinoids (THCA, CBGA, and CBDA)—was used to identify the Italian retailers of ''[[wikipedia:Cannabis sativa|Cannabis sativa]]'' L. ([[wikipedia:Hemp|hemp]]), reinforcing the idea that the practice of categorizing hemp samples only using THC and CBD is inadequate. A [[high-performance liquid chromatography]]–[[tandem mass spectrometry]] (HPLC-MS/MS) method was developed for screening and simultaneously analyzing the nine cannabinoids in 161 hemp samples sold by four retailers located in different Italian cities. The hemp samples dataset was analyzed by [[wikipedia:Univariate analysis|univariate]] and [[wikipedia:Multivariate analysis|multivariate analysis]], with the aim to identify the associated hemp retailers without using any other [[information]] on the hemp samples such as [[wikipedia:Cannabis strains|''Cannabis'' strains]], seeds, soil and cultivation characteristics, geographical origin, product storage, etc. The univariate analysis highlighted that the hemp samples could not be differentiated by using any of the nine cannabinoids analyzed. ('''[[Journal:Identification of Cannabis sativa L. (hemp) retailers by means of multivariate analysis of cannabinoids|Full article...]]''')<br />
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[[wikipedia:Cannabis|Cannabis]] has regained much attention as a result of updated legislation authorizing many different uses, and it can be classified on the basis of the content of [[wikipedia:Tetrahydrocannabinol|Δ9-tetrahydrocannabinol]] (Δ9-THC), a psychotropic substance for which there are legal limitations in many countries. For this purpose, accurate qualitative and quantitative determination is essential. The relationship between THC and [[wikipedia:Cannabidiol|cannabidiol]] (CBD) is also significant, as the latter substance is endowed with many specific and non-psychoactive proprieties. For these reasons, it becomes increasingly important and urgent to utilize fast, easy, validated, and harmonized procedures for determination of [[wikipedia:Cannabinoid|cannabinoids]]. The procedure described herein allows rapid determination of 10 cannabinoids from the [[wikipedia:Inflorescence|inflorescences]] of ''Cannabis sativa'' L. by extraction with organic solvents. Separation and subsequent detection are by [[wikipedia:Reversed-phase chromatography|reversed-phase]] [[high-performance liquid chromatography]] with ultraviolet detector (RP-HPLC-UV). ('''[[Journal:Fast detection of 10 cannabinoids by RP-HPLC-UV method in Cannabis sativa L.|Full article...]]''')<br />
 
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Revision as of 16:43, 20 January 2020

Fig1 Mandrioli Molecules2019 24-11.png

"Fast detection of 10 cannabinoids by RP-HPLC-UV method in Cannabis sativa L."

Cannabis has regained much attention as a result of updated legislation authorizing many different uses, and it can be classified on the basis of the content of Δ9-tetrahydrocannabinol (Δ9-THC), a psychotropic substance for which there are legal limitations in many countries. For this purpose, accurate qualitative and quantitative determination is essential. The relationship between THC and cannabidiol (CBD) is also significant, as the latter substance is endowed with many specific and non-psychoactive proprieties. For these reasons, it becomes increasingly important and urgent to utilize fast, easy, validated, and harmonized procedures for determination of cannabinoids. The procedure described herein allows rapid determination of 10 cannabinoids from the inflorescences of Cannabis sativa L. by extraction with organic solvents. Separation and subsequent detection are by reversed-phase high-performance liquid chromatography with ultraviolet detector (RP-HPLC-UV). (Full article...)

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