Difference between revisions of "User:Shawndouglas/sandbox/sublevel36"

From LIMSWiki
Jump to navigationJump to search
Line 17: Line 17:
* https://www.techexplorist.com/improving-speed-gold-standard-covid-19-diagnostic-test/34069/
* https://www.techexplorist.com/improving-speed-gold-standard-covid-19-diagnostic-test/34069/
* https://www.sciencemag.org/news/2020/08/radical-shift-testing-strategy-needed-reopen-schools-and-businesses-researchers-say
* https://www.sciencemag.org/news/2020/08/radical-shift-testing-strategy-needed-reopen-schools-and-businesses-researchers-say
* https://asiatimes.com/2020/08/pooled-virus-tests-help-stretched-health-services/
* https://www.healthline.com/health-news/how-pooled-testing-can-help-us-fight-spread-of-covid-19
* https://onlinelibrary.wiley.com/doi/full/10.1002/jmv.26328
* https://onlinelibrary.wiley.com/doi/full/10.1002/jmv.26328
* https://www.fiercebiotech.com/medtech/oxford-researchers-develop-portable-covid-19-test-costing-less-than-25
* https://www.fiercebiotech.com/medtech/oxford-researchers-develop-portable-covid-19-test-costing-less-than-25
Line 25: Line 27:


* https://thejewishvoice.com/2020/08/10-ways-israeli-scientists-are-improving-corona-testing-part-1/
* https://thejewishvoice.com/2020/08/10-ways-israeli-scientists-are-improving-corona-testing-part-1/
* https://www.nation.co.ke/kenya/news/scientists-develop-covid-19-rapid-test-kit-1912962
Alternative reagents:
* https://www.engineeringnews.co.za/article/faster-local-covid-19-test-kits-could-be-ready-by-year-end-2020-07-30/rep_id:4136
POLs:
* https://hitconsultant.net/2020/08/03/talis-nih-contract-for-point-of-care-covid-19-testing/
* https://www.nih.gov/news-events/news-releases/nih-delivering-new-covid-19-testing-technologies-meet-us-demand


===3.1 The equipment===
===3.1 The equipment===
====Instruments====
====Instruments====
[[File:Eppendorf Mastercycler Pro S.jpg|right|400px|thumb|Eppendorf Mastercycler Pro S, a thermal cycler for PCR and other applications]] Thermal cyclers (a.k.a. PCR machines) - standard PCR systems, RT PCR -  advantages of digital PCR systems such as precision, sensitivity, accuracy, reproducibility, direct quantification and multiplexing, and speed of the analysis systems, and digital PCR systems "The market is witnessing an emerging trend of digital and droplet digital PCR technology, which is sensitive and accurate than the traditional method."
[[File:Eppendorf Mastercycler Pro S.jpg|right|400px|thumb|Eppendorf Mastercycler Pro S, a thermal cycler for PCR and other applications]] Thermal cyclers (a.k.a. PCR machines) - standard PCR systems, RT PCR -  advantages of digital PCR systems such as precision, sensitivity, accuracy, reproducibility, direct quantification and multiplexing, and speed of the analysis systems, and digital PCR systems "The market is witnessing an emerging trend of digital and droplet digital PCR technology, which is sensitive and accurate than the traditional method."
https://www.thermofisher.com/search/browse/category/us/en/602552/PCR+Machines+%28Thermal+Cyclers%29+%26+Accessories
https://blog.biomeme.com/how-do-you-test-for-covid-19


====Reagents====
====Reagents====
Line 35: Line 50:
====Consumables====
====Consumables====
PCR tubes, plates, and other accessories
PCR tubes, plates, and other accessories
https://www.sigmaaldrich.com/labware/labware-products.html?TablePage=9577275


====Software and services====
====Software and services====

Revision as of 21:28, 5 August 2020

3. Adding COVID-19 and other virus testing to your laboratory

In the previous chapter, the most common testing methodologies for COVID-19 and other coronaviruses was discussed in detail. The common thread among them all was the use of real-time reverse-transcription polymerase chain reaction (rRT-PCR) assays for testing.


"The PCR technique has been found to be useful in pharmaceutical and biotechnology research activities as well as microbial quality testing. The technique is also applied in genetic engineering. It is used to identify genes related to certain phenotypes including genetic disorders. Regular testing of the microbial load of raw materials and finished products is an important process in the pharmaceutical & biotechnology industry. Sophisticated analytical methods such as polymerase chain reaction (PCR) have been widely applied for quality control analysis in the pharmaceutical sector."

"Continuous technology advancements are making it more efficient and user-friendly, but the high cost of the instruments, servicing contracts, and reagents pose major challenges for the market, especially to the price-sensitive academics. Currently, the market players are involved in enhancing the capabilities of the PCR systems, and increasing its specificity and sensitivity."

The explosion of new coronavirus tests that could help to end the pandemic https://www.nature.com/articles/d41586-020-02140-8

Methods for speeding up PCR (and alternatives such as LAMP and CRISPR):

Alternative testing:

Alternative reagents:

POLs:


3.1 The equipment

Instruments

Eppendorf Mastercycler Pro S, a thermal cycler for PCR and other applications

Thermal cyclers (a.k.a. PCR machines) - standard PCR systems, RT PCR - advantages of digital PCR systems such as precision, sensitivity, accuracy, reproducibility, direct quantification and multiplexing, and speed of the analysis systems, and digital PCR systems "The market is witnessing an emerging trend of digital and droplet digital PCR technology, which is sensitive and accurate than the traditional method."

https://www.thermofisher.com/search/browse/category/us/en/602552/PCR+Machines+%28Thermal+Cyclers%29+%26+Accessories https://blog.biomeme.com/how-do-you-test-for-covid-19

Reagents

template DNA, PCR primers and probes, dNTPs, PCR buffers, enzymes, and master mixes

Consumables

PCR tubes, plates, and other accessories https://www.sigmaaldrich.com/labware/labware-products.html?TablePage=9577275

Software and services

3.2 The vendors

Major players operating in the global PCR market are Bio-Rad Laboratories, Inc., QIAGEN N.V., F. Hoffmann-La Roche AG, Thermo Fisher Scientific, Inc. Becton, Dickinson and Company, Abbott, Siemens Healthcare GmbH (Siemens AG), bioMérieux SA, Danaher Corporation, and Agilent Technologies. Merck KGaA, Promega


References