Difference between revisions of "ELISA"

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Enzyme Linked Immunosorbent [[Assay]]s. A diagnostic test utilizing an enzyme-labeled immunoreactant (antigen or antibody) and an Immunosorbent (antigen or antibody bound to a solid support). Also called EIA.
'''The enzyme-linked immunosorbent assay''' ('''ELISA''') is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.<ref name="LequinELISA">{{cite journal |url=http://www.clinchem.org/content/51/12/2415 |title=Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA) |author=Lequin, Rudolf M. |journal=Clinical Chemistry |volume=51 |issue=12 |pages=2415–8 |year=2005 |pmid=16179424 |doi=10.1373/clinchem.2005.05153 |accessdate=03 August 2014}}</ref>
 
==References==
<references />

Revision as of 17:23, 3 August 2014

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.[1]

References

  1. Lequin, Rudolf M. (2005). "Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)". Clinical Chemistry 51 (12): 2415–8. doi:10.1373/clinchem.2005.05153. PMID 16179424. http://www.clinchem.org/content/51/12/2415. Retrieved 03 August 2014.