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In the laboratory world, a spike, as defined by the United States Environmental Protection Agency via its SEDD Specification Version 5.2, is "a reanalysis of a regular sample done for quality control purposes with known amounts of target analytes added to the sample." The EPA adds that the "data is expected to include an original client sample ID for each sample plus method node and, potentially, a percent recovery and an expected result for each analyte for which the analyte type is a spike. The original client sample ID identifies the original sample that was spiked."[1]


  1. U.S. Environmental Protection Agency (March 2019). "Appendix A. Data Element Dictionary (DED)" (PDF). SEDD Specification Version 5.2. https://www.epa.gov/sites/default/files/2019-05/documents/sedd_spec_v5-2-march_2019_508.pdf. Retrieved 22 September 2022.