Journal:Laboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis
| Full article title | Laboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis |
|---|---|
| Journal | Journal of Microbiology, Immunology and Infection |
| Author(s) | Jing, Ran; Kudinha, Timothy; Zhou, Meng-Lan; Xiao, Meng; Wang, He; Yang, Wen-Hang; Xu, Ying-Chun; Hsueh, Po-Ren |
| Author affiliation(s) |
Chinese Academy of Medical Sciences, Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Charles Sturt University, NSW Health Pathology, National Taiwan University College of Medicine |
| Primary contact | Email: tkudinha at yahoo dot com |
| Year published | 2020 |
| Volume and issue | In Press |
| DOI | 10.1016/j.jmii.2020.10.004 |
| ISSN | 1684-1182 |
| Distribution license | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
| Website | https://www.sciencedirect.com/science/article/pii/S1684118220302498 |
| Download | https://www.sciencedirect.com/science/article/pii/S1684118220302498/pdfft (PDF) |
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This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
Since the initial emergence of coronavirus disease 2019 (COVID-19) in Wuhan, Hubei province, China, a rapid spread of the disease occurred around the world, becoming an international global health concern at the pandemic level. In the face of this medical challenge threatening humans, the development of rapid and accurate methods for early screening and diagnosis of COVID-19 became crucial to containing the emerging public health threat, and preventing further spread within the population. Despite the large number of COVID-19 confirmed cases in China, some problematic cases with inconsistent laboratory testing results were reported. Specifically, a high false-negative rate of 41% on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection by real-time reverse transcription polymerase chain reaction (qRT-PCR) assays was observed in China. Although serological testing has been applied worldwide as a complementary method to help identify SARS-CoV-2, several limitations on its use have been reported in China. Therefore, the separate use of qRT-PCR and serological testing in the diagnosis of COVID-19 in China and elsewhere presents considerable challenges, but when used in combination, these methods can be valuable tools in the fight against COVID-19. In this review, we give an overview of the advantages and disadvantages of different molecular techniques for SARS-CoV-2 detection that are currently used in several labs, including qRT-PCR, gene sequencing, loop-mediated isothermal amplification (LAMP), nucleic acid mass spectrometry (MS), and gene editing techniques based on the clustered regularly interspaced short palindromic repeats (CRISPR/Cas13) system. Then we mainly review and analyze some causes of false-negative qRT-PCR results, and how to resolve some of the diagnostic dilemmas.
Keywords: SARS-CoV-2, COVID-19, qRT-PCR, serology testing, challenging cases
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This presentation is faithful to the original, with only a few minor changes to presentation, spelling, and grammar. In some cases important information was missing from the references, and that information was added. Otherwise, no other changes were made in conformance with the "NoDerivatives" portion of the document license.
